Post-doc in Transcription Factor Condensation Research

Institution IMB (www.imb.de) is a research institute on the campus of Johannes Gutenberg University in Mainz, Germany. It is generously funded by the Boehringer Ingelheim Foundation and the state of Rhineland-Palatinate. Our research focuses on the biology of the cell nucleus and ranges from the molecular level to systems and computational approaches. Researchers at IMB are supported by strong core facilities that offer state-of-the-art equipment and training in protein production, microscopy, proteomics, cytometry, genomics and bioinformatics., Position As part of the Protein Disorder in Transcription group headed by Sina Wittmann, you will work on a project that studies condensation of transcription factors on DNA. Thanks to the development of new imaging techniques, recent years uncovered that the transcription initiation machinery condenses into droplets with liquid-like properties. The lab is generally interested in understanding how these transcriptional condensates are formed and how they regulate gene expression (for more information visit www.imb.de/research/our-research-groups/wittmann/research ). Biological condensation is often driven by IDRs, protein regions that do not fold into a 3D structure but remain flexible. Strikingly, IDRs are highly over-represented in proteins involved in transcription, especially in transcription factors. We are trying to decipher how IDRs control the formation and properties of condensates with approaches that range from in vitro biochemistry to genome-wide expression and binding studies in cells. In your project, we do not merely want to study bulk phenomena but decipher how the IDR composition controls the formation of transcription factor assemblies on the molecular level. For this, we are trying to determine how many molecules are contained within an assembly and what their molecular arrangement is. Furthermore, transcription factors assemble on DNA in vivo and you will specifically look at the role of DNA in the assembly

Ihre Aufgaben

process. To this end, you will use our brand-new Lumicks optical tweezers system, perform state-of-the-art microscopy, DNA curtain assays, recombinant expression and purification of proteins, phase separation and DNA binding assays as well as analysis of all data generated. The project is part of the collaborative research centre (CRC) 1551 which is a multi-disciplinary endeavour to understand 'polymer concepts in cellular function'. As such, the project is a collaboration with the groups of Edward Lemke and Martin Girard, both located on the same campus.

Responsibilities

• Characterisation of transcription factor assemblies using both bulk assays (fluorescence microscopy, scattering) and singlemolecule assays (optical tweezers and DNA curtains)
• Generation of different DNA substrates
• Recombinant expression and purification of proteins
• Analysis and documentation of all data generated
• Regular participation and presentation at CRC1551 events which involves presenting to and discussing with scientists from other disciplines (physics, chemistry, computational biology)
• Regular participation and presentation in meetings at IMB and in conferences
• Study of relevant scientific literature

Ihr Profil

• Recently completed PhD in biochemistry, structural biology or related discipline
• Strong background in biochemistry and biophysical assays
• Ability to work closely with scientists outside your field (esp. physicists)
• Experience in recombinant expression and purification of proteins as well as cloning
• Ability to work precisely and good attention to detail
• Ability to work independently as well as in a team
• Willingness to learn new skills
• Very good oral and written communication skills in English
• Experience in the following is desired: o working in an inter-disciplinary environment o using optical tweezers or other single-molecule technique o experimentation with IDR-containing proteins o fluorescence microscopy o python (or other language) for data analysis o background in the regulation of gene expression

Wir bieten Ihnen

campus with many events relevant for your research and career progression. Our offer includes

• Competitive salary and favourable pension scheme
• Advanced training opportunities
• Flexible working hours
• Employee events
• Job Ticket
• Fully integrated into the CRC1551 (more info about CRC1551 here)
• Travel money
• Optional Winter School
• Joint meeting with SPP2191
• Mentorship program
• Regular seminars with world class speakers

Kontakt

What else you need to know

• Starting Date: February 2025 or later
• Duration: initially 2 years with possibility of extension
• Deadline: 15th December 2024
• International applicants: we will assist you through the visa process, and also with your application for a residence/work permit after your arrival in Mainz. Have we sparked your interest: To apply, please upload your application containing your cover letter, CV, certificates and contact information of at least two professional references to our application form on imb.de/jobs/scientific-positions. Should you have any questions regarding the application process, please contact us by email hr@imb-mainz.de, quoting Ref. No. #SWPD01. Informal enquires should be addressed to Dr Sina Wittmann. IMB is an equal opportunity employer.

Contact Apply on our application form on imb.de/jobs/scientificpositions. Should you have any questions regarding the application process, please contact us by email hr@imbmainz.de, quoting Ref. No. #SWPD01.